Bacteria with a Thick Cell Wall(20 - 40 nm) absorb the Dye and are stained purple, and are defined
as Gram - Positive. Bacteria with a Thin
Cell Wall(2 - 7nm) absorb only a Small Amount of
Dye, which is washed out by Acetone. These Bacteria are stained Pink with a Second Dye
and
are said to be Gram - Negative. Although Gram - Negative Bacteria have a Thin Cell
Wal, they have an additional outer Membrane not
present in Gram - Positive Bacteria.
This outer Membrane is made of Lipopolysaccharides - similar in character to the Cell
Membrane.
These differences in Cell walls and Membranes have important consequences for the different
vulnerabilities of Gram - Positive and
Gram - Negative Bacteria to AnbtiBacterial Drugs.
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